9:45 AM - 10:00 AM
[BPT04-04] Transcript assembly and quantification identify candidate genes for foraminiferal calcification
Keywords:calcification, single-cell eukaryote, gene expression
In order to reveal biomineralizing mechanism of foraminifers, we here present the gene expression profiles during the foraminiferal calcification for the first time. We used cultured specimens of Ammonia beccarii, which have hyaline calcite, and applied comparative transcriptomic survey by using high-throughput mRNA sequencing (RNA-seq). We performed de novo assembly using ~67 million pair-end reads of mRNAs, and then mapped back the reads to the open read frames (ORFs) for calculating the expression levels. From the results, we identified the candidate genes for the calcification process; Ca2+ is transported through multiple ion channels from an extracellular region and pooled in vesicles. These Ca2+ seem to play important role for mitochondrial ATP synthesis. We also found the genes coding enzymes, which are related to generate bicarbonate in a cell and to release it to extracellular. In taking account of the studies of cell observations, the present study figured out transportations of ions and molecules for calcification.