[MIS10-P04] Visualization of local environment around forming/dissolving carbonate minerals with the fluorescent probe
Keywords:Visulization, carbonate minerals, pH
Calcium carbonate was synthesized by the counter diffusion method, in which calcium chloride and sodium carbonate solutions were counter-diffused through an agarose gel matrix containing the fluorescent probe like 8-hydroxypyrene-1,3,6-trisul-fonic acid (HPTS). We observed under a microscope that pH in the middle part of gel first increased gradually with time and turn downward after crystals formed. The counter diffusion method with gel media for the carbonate synthesis has attracted attention as a valid tool in the study of biomineralization because the calcification sites in some organisms were recently suggested to have the features of a highly viscous sol or gel matrix. Therefore, the detail observation of the local condition around forming calcium carbonate in gel matrix could provide new insights into the knowledge of biomineralization mechanisms.