Japan Geoscience Union Meeting 2019

Presentation information

[J] Oral

M (Multidisciplinary and Interdisciplinary) » M-IS Intersection

[M-IS13] Biogeochemistry

Mon. May 27, 2019 1:45 PM - 3:15 PM 201A (2F)

convener:Keisuke Koba(Center for Ecological Research, Kyoto University), Hideaki Shibata(Field Science Center fot Northern Biosphere, Hokkaido University), Naohiko Ohkouchi(Japan Agency for Marine-Earth Science and Technology), Youhei Yamashita(Faculty of Environmental Earth Science, Hokkaido University), Chairperson:Keisuke Koba(Kyoto University), Yohei Yamashita, Kazuya Nishina, Kazumichi Fujii

1:45 PM - 2:00 PM

[MIS13-07] Ultrahigh-resolution mass spectral analysis of dissolved organic matter in rainfall, throughfall and soil water in temperate forest stands, western Japan

*Junichiro Ide1, Keitaro Yamase2, Seonghun Jeong3, Noki Makita4, Hiroshi Nishimura5, Keitaro Fukushima6, Kyoichi Otsuki7, Mizue Ohashi8 (1.Institute of Decision Science for a Sustainable Society, Kyushu University, 2.Forestry and Forest Products Research Institute, Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries, 3.Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, 4.Faculty of Science, Shinshu University, 5.Research Institute for Sustainable Humanosphere, Kyoto University, 6.The Center for Ecological Research, Kyoto University, 7.Faculty of Agriculture, Kyushu University, 8.School of Human Science and Environment, University of Hyogo)

Keywords:Biomolecules, Carbon dynamics, Forest type, FT-ICR MS, Molecular diversity

This study aimed to clarify alternations of the quality of dissolved organic matter (DOM) in rainwater moving through temperate forests. For this, we compared the molecular composition of dissolved organic matter (DOM) between bulk deposition (rainfall), throughfall and soil water in Japanese cypress (Cryptomeria japonica) and Japanese stone oak (Lithocarpus edulis) stands, which were regarded as coniferous and broad-leaved stands, respectively, during a rain event. Analyses of ultrahigh-resolution Fourier transform ion cyclotron resonance mass spectrometry revealed that the number of DOM molecular species detected was significantly greater in throughfall and soil water than in rainfall (p < 0.05). In contrast, the number of molecular species did not significantly differ between throughfall and soil water (p > 0.05). There were no significant differences in the number of molecular species in both throughfall and soil water between coniferous and broad-leaved stands (p > 0.05). These results suggest that a diverse array of DOM molecules are provided from the tree canopies and soils to the rainwater in both stands. Approximately 52%–59% of the total molecular species in throughfall samples (n = 6) were unique to throughfall. Additionally, approximately a half of the total molecular species in soil water samples (n = 5) were unique to soil water. The classification of molecular species into seven biomolecular classes using a van Krevelen diagram revealed that throughfall samples contained many of lipid and protein molecules compared to rainfall and soil water samples, whereas approximately 70% of the total molecular species in soil water were lignin-like molecules. The analysis of multidimensional scaling revealed that molecular species were significantly differentiated between rainfall, throughfall and soil water (p < 0.05). These results indicate that there are diverse molecules unique to throughfall and soil water and that the quality of DOM drastically changes in rainwater as it moves through temperate forests.