[BBC03-P05] The rediscovery of diversity of archaeol derivative in halophilic archaea especially living in high salt concentration
Keywords:halophilic archaea, archaeol, diversity, ether lipid
Archaea has a characteristic lipid-core, archaeol(1). Further, a characteristic diether lipid-core (C20-C25 diether (2), extended archaeol) which is constructed from one C25 and one C20 isoprenoid is produced by halophilic archaea[1]. Recently, Dawson et al. showed the existence of several unsaturated isoprenoid diethers in the lipid-core of several halophilic archaea isolated from very high salt concentration[2]. On the other hands, existence of regioisomer 3 were shown in halite from Spain[3].
In my study, chemically synthesized unsaturated diether were different from those of Dawson’s unsaturated diether, the existence of the regioisomeric mixture were strongly suggested[4]. And the chemical synthesis of 2 and 3 revealed diether in halite is the mixture of 2 and 3[5].
Until now, 2 have been the only isomer from a cultured microorganic origin. On the other hand, 2 and 3 were existed in halite. Further, the comparison of chemical synthetic unsaturated diether and microorganic samples, diversity of unsaturation in archaeol and extended archaeol were observed in perliminary study. The search of 3, and the analysis of diversity of unsaturation of archaeol and extended archaeol were conducted in each microorganisms, the halophilic archaea especially living in a high salt concentration. The comprehensive analysis of lipid core obtained from the type cultures in Riken BRC, isolated from high salt concentration field, or from halite were incubated, and the lipid-core analysis were executed. The progress of this study is presented.
About the the preparation of lipid core, alkaline hydrolysis of polar head group used by the preparation ofhydroxyarchaeol[6] were adopted instead of acid hydrolysis usually performed. Thus, the divirsity of unsaturtion of archaeol derivative in each halophilic archaea species. And the exsitence of extended archaeol isomer 3 from the Halorhabus utahensis (JCM 11049) with its unsaturated derivative were proved. So far, the archaeol derivative were only discussed in 1, 2 and its unsatutaed isomers. However, the relatively large diversity in each species in extremely halophilic archaea exists. Further studies from the comprehensive analysis is in progress.
[1] De Rosa et al., J. Gen Microbiol (1982) 265: 343. [2] Dawson et al., Org Geochem (1982) 48: 1. [3] Teixidor et al., Geochem Cosmochim Acta (1993) 57: 4479. [4] Yamauchi, Res Org Geochem (2019) 35: 1. [5] Yamauchi, (2019) JpGU meeting 2019, BBG02-P03. [6] Sprott et al., J Biol Chem (1990) 265: 13735.
In my study, chemically synthesized unsaturated diether were different from those of Dawson’s unsaturated diether, the existence of the regioisomeric mixture were strongly suggested[4]. And the chemical synthesis of 2 and 3 revealed diether in halite is the mixture of 2 and 3[5].
Until now, 2 have been the only isomer from a cultured microorganic origin. On the other hand, 2 and 3 were existed in halite. Further, the comparison of chemical synthetic unsaturated diether and microorganic samples, diversity of unsaturation in archaeol and extended archaeol were observed in perliminary study. The search of 3, and the analysis of diversity of unsaturation of archaeol and extended archaeol were conducted in each microorganisms, the halophilic archaea especially living in a high salt concentration. The comprehensive analysis of lipid core obtained from the type cultures in Riken BRC, isolated from high salt concentration field, or from halite were incubated, and the lipid-core analysis were executed. The progress of this study is presented.
About the the preparation of lipid core, alkaline hydrolysis of polar head group used by the preparation ofhydroxyarchaeol[6] were adopted instead of acid hydrolysis usually performed. Thus, the divirsity of unsaturtion of archaeol derivative in each halophilic archaea species. And the exsitence of extended archaeol isomer 3 from the Halorhabus utahensis (JCM 11049) with its unsaturated derivative were proved. So far, the archaeol derivative were only discussed in 1, 2 and its unsatutaed isomers. However, the relatively large diversity in each species in extremely halophilic archaea exists. Further studies from the comprehensive analysis is in progress.
[1] De Rosa et al., J. Gen Microbiol (1982) 265: 343. [2] Dawson et al., Org Geochem (1982) 48: 1. [3] Teixidor et al., Geochem Cosmochim Acta (1993) 57: 4479. [4] Yamauchi, Res Org Geochem (2019) 35: 1. [5] Yamauchi, (2019) JpGU meeting 2019, BBG02-P03. [6] Sprott et al., J Biol Chem (1990) 265: 13735.