[MIS25-P05] Stable carbon and nitrogen isotope analysis of bacterial nucleic acid
Keywords:Stable isotope, Nucleic acid
Nucleic acids were extracted from incubated denitrifying bacteria (Pseudomonas aureofaciens) using reagent for nucleic acid extraction, ISOPLANT and ISOPLANT II (Nippon Gene Co., Ltd.). The extractions were precipitated under ethanol, and then were dissolved in water. A part of the solution was used for protein, RNA, and DNA concentrations, and the remain was used for isotope analysis.
The extraction by ISOPLANT II contained a large amount of protein and contaminant, which suggest that the reagent is inadequate foe isotope analysis. On the other hand, protein was not detected in the extraction by ISOPLANT. The extraction almost consists of RNA rather than DNA. Offsets of carbon and nitrogen isotope ratios between biomass and the extraction by ISOPLANT insignificantly varied, which imply that the isotope ratios of bacterial RNA are controlled by those of biomass.