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[MIS14-P03] Determination on the Triple Oxygen Isotopic Composition of Nitrous Oxide Emitted from Soils
Keywords:Nitrous Oxide, Triple Oxygen Isotopic Composition, Nitrification, Denitrification, Soil
N2O produced via nitrification pathway has oxygen atom originates from atmospheric oxygen molecules (O2: Δ17O = –0.4 ‰) while the oxygen atom of N2O produced via denitrification pathway originates from nitrate (NO3-: Δ17O > 0 ‰) or water (H2O: Δ17O = 0 ‰). Therefore, it is expected that the N2O production pathways could be elucidated from the Δ17O value. In this study, we quantified the Δ17O of N2O emitted from soils, the forest areas in Gifu and Aichi prefectures and the tea plantations in Mie prefecture, to determine the contribution (mixing ratio) of the nitrification and denitrification. In the forest area of Gifu prefecture, we collected soil N2O accumulated in snow. In Aichi and Mie prefectures, N2O emitted from soils were collected using an open-flow chamber. In addition, in order to confirm the effect of atmospheric NO3-(Δ17O = 26.3 ± 3 ‰) on the N2O production in soil ecosystems during the raining events, we also performed chamber experiments both during and after the natural raining events.
The Δ17O values of N2O emitted from the forested soils, both in Gifu and Aichi prefecture, were -0.4 ‰, implying that oxygen atom in N2O originates from atmospheric O2 via microbial nitrification. On the other hand, the Δ17O value of N2O emitted from tea plantation field was -0.2‰, implying that the oxygen atom in N2O derives from O2, H2O and soil NO3-(Δ17O = +0.5 ‰) via both nitrification and denitrification. The Δ17O value of N2O emitted from forested soils in campus (Aichi prefecture) during the natural raining days reached up to +5 ‰, implying that all the oxygen atoms in N2O were derived from NO3-, and was probably derived directly from part of the atmospheric NO3-via denitrification in the surface soils. We concluded that the main microbial processes to produce N2O within the soil ecosystems have been changed from nitrification to denitrification during the raining events.