[M-ZZ47] Marine Manganese Deposits - Genesis, environment, and development -

[MZZ47-P03] Genetic population structure of deep-sea benthos in a potential marine mineral resource area

*Hiroki Kise¹, AKIRA IGUCHI^1,2, TAKUJI IKEGAMI³, YOSUKE ONISHI³, KOICHI GOTO³, YUICHIRO TANAKA¹, TRAVIS W. WASHBURN¹, MIYUKI NISHIJIMA¹, TAIGA KUNISHIMA^1,4, SHOGO KATO⁵, TAKAAKI MATSUI⁶, NOBUYUKI OKAMOTO⁷, ATSUSHI SUZUKI^1,2 (1.Geological Survey of Japan, National Institute of Advanced Industrial Science and Technology (AIST), 2.Research Laboratory on Environmentally-conscious Developments and Technologies [E-code], National Institute of Advanced Industrial Science and Technology (AIST), 3.KANSO CO., LTD, 4.Wakayama Prefectural Museum of Natural History, 5.IDEA Consultants, Inc., 6.Marine Biological Research Institute of Japan CO., LTD, 7.Japan Oil, Gas and Metals National Corporation (JOGMEC))

Keywords:Deep-sea mineral resources, Benthos, DNA barcoding, genetic population structure

The exploitation of deep-sea mineral resources has attracted much attention in recent years, and there is a need to conduct environmental baseline surveys related to this issue. In this study, we aimed to clarify the basic information on the population structure of deep-sea benthos found in a potential marine mineral resource area in the Northwest Pacific Ocean by molecular genetic analyses. DNA barcoding analysis with mitochondrial cytochrome c oxidase subunit I gene region and population genetic analysis were conducted for the dominant species collected in the environmental baseline surveys. In the case of synaphobranchid eels, high genetic diversity was observed in each population, and no significant genetic differentiation was detected among populations, suggesting that high connectivity is maintained among populations. Single nucleotide polymorphism (SNP) analysis using Simenchelys parasitica confirmed the high degree of connectivity among the populations. As for the amphipods, molecular phylogenetic analysis revealed several single putative species and some with low genetic diversity. We also confirmed that high genetic diversity was maintained for one decapod species. The results of this study provide important fundamental insights into the genetic population structure of deep-sea benthos found in potential areas for marine mineral exploitation, and will be useful for estimating the effects of disturbances on populations and for selecting candidate species for future connectivity analyses.