Japan Geoscience Union Meeting 2024

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[E] Oral

B (Biogeosciences ) » B-CG Complex & General

[B-CG05] Frontier in diversity and ecology of protists and microfossils

Fri. May 31, 2024 9:00 AM - 10:15 AM 304 (International Conference Hall, Makuhari Messe)

convener:Rie Hori, S.(Department of Earth Science, Faculty of Science, Ehime University), Yurika Ujiie(Kochi University), Yasuhide Nakamura(Estuary Research Center, Shimane University), Peter Oliver Baumgartner(University of Lausanne), Chairperson:Yurika Ujiie(Kochi University), Rie Hori, S.(Department of Earth Science, Faculty of Science, Ehime University)


9:45 AM - 10:00 AM

[BCG05-04] Physiologic and metabolic changes on Foraminifera when exposed to nanoplastics

*Yoshiyuki Ishitani1, Caterina Ciacci2, Yurika Ujiie3, Hidetaka Nomaki1, Fabrizio Frontalini2 (1.Japan Agency for Marine-Earth Science and Technology, 2.University of Urbino, 3.Kochi University)

Keywords:Nanoplastics, Foraminifera, Confocal laser scanning microscopic observations, comparative transcriptome analyses

Plastic pollution is one of the most urgent environmental issues facing us today. Plastic debris are persistently discharged into the ocean and are broken into micro-to-nano sized pieces through drifting along ocean gyre, then spread all over the oceans. Nano-sized plastics (nanoplastics) have considerably more toxic effects on marine organisms compared to large-sized plastics because their increased surface area enhance adsorption and leaching of toxic substances for organisms. Two kinds of functionalized nanoplastics, namely carboxylated- (PS-COOH) and cationic amine- (PS-NH2) terminated polystyrene, are known to induce adverse effects on marine organisms. These nanoplastics lead to harmful effects on physiology as well as cellular alterations such as intensified productions of reactive oxygen species (ROS) and lipidic polarity changes. However, the underlying mechanisms and metabolic pathways of nanoplastic toxicity remain largely unknown. Here, we tackle these gaps through a time-course (1, 6 and 24-hours) exposure experiment with two characteristic (PS-COOH and PS-NH2) nanoplastics using the benthic foraminiferal strain, Ammonia veneta. The confocal laser scanning microscopic (CLSM) observations with three specific probes (CellROX@Green for ROS, Acridine Orange for normal and acid endosome, and Nile Red for polar and neutral lipid) showed different patterns in ROS and lipidic polarity between two nanoplastics. The time-course transcriptome analyses based on single-cell RNA-seq identified genes related to toxicity, and unveiled their metabolic pathways.