[B-CG05] Methane in terrestrial and aquatic ecosystems: from microbes to the atmosphere

[BCG05-P04] Methane mitigation in a rice pot experiment: effects of irrigation and modified psy2-3 gene in kitaake rice (Oryza Sativa subsp. japonica)

*Hao-Chi Lin¹, Charles C.-K. Chou¹, Kuo-Chen Yeh², Yi-Ying Chen¹, Swee-Suak Ko³ (1.Research Center for Environmental Changes (RCEC), Academia Sinica, Taipei, Taiwan, 2.Agricultural Biotechnology Research Center (ABRC), Academia Sinica, Taipei, Taiwan, 3.Agricultural Biotechnology Research Center (ABRC), Academia Sinica south campus, Tainan, Taiwan)

Keywords:alternate wetting and drying, kitaake, methane, pot experiment, PSY2-3

Rice cultivation sustains nearly half of the global population but significantly contributes to atmospheric methane (CH₄) emissions. To mitigate these emissions in rice paddies, we estimated CH₄ flux using an automatic closed-chamber system in a pot experiment conducted under continuous flooding (CF) and alternate wetting and drying (AWD) irrigation treatments in a greenhouse at Academia Sinica (Taipei, Taiwan). We used both a japonica rice (Kitaake) and a modified Kitaake genotype containing plant peptides with sulfated tyrosine 2-3 (PSY2-3). Our findings demonstrated the 50% reduction in CH₄ flux under the AWD treatments. Furthermore, the modified PSY2-3 genotype exhibited a significant 10 to 30% lower CH₄ emission compared to the wild type after tillering stage (around 7^th weeks) due to aerenchyma tissue development. These results revealed the importance of both irrigation management and physiological differences in mitigating CH₄ emissions from rice paddies.