5:15 PM - 7:15 PM
[MIS22-P05] Soil property and colonization of arbuscular mycorrhizal fungi in Sasa species in serpentine soils
Keywords:Arbuscular mycorrhizal fungi, community structure, serpentine soils, Sasa senanensis, Sasa kurilensis, heavy metal content
Arbuscular mycorrhizal fungi (AMF) play an essential role in enhancing nutrient absorption and improving stress tolerance in plants. They also have potential value in environmental remediation. Serpentine soils are rich in heavy metals such as Ni, Cr, and Mg and lack essential nutrients, which negatively affects plant growth. In this regard, AMF may play a promising role in promoting plant growth in serpentine soils. This study aims to evaluate the difference in the soil property , the infection rates of AMF in Sasa senanensis and Sasa kurilensis, as well as community structure of AMF in Sasa species and in soil serpentine rock area and non-serpentine rock (sedimentary rock) areaand to clarify the influence of soil factors on these variables.
The survey was conducted at the Nakagawa and Tesio Experimental Forests of Hokkaido University located in northern Hokkaido. In October 2024, twelve plots were established in adjacent serpentine and non-serpentine rock (sedimentary rock) areas, and the aboveground and belowground parts of Sasa senanensis or Sasa kurilensis grown there, as well as soil up to a depth of 10 cm were collected. Three replicates were taken from each plot. Soil samples were analyzed for pH, electrical conductivity (EC), soil moisture content, total carbon content (TC), and total nitrogen content(TN). Available nitrogen, phosphate, extractive organic nitrogen, extractive organic phosphorus, and heavy metal concentrations (Fe, Zn, Cu, Cr, Ni, Mn, Mg) in the soil were also measured. The collected fine roots were stained, and the colonization levels of AMF were assessed under a microscope. DNA was extracted from both soil and roots, and ITS and 28S rDNA were amplified with the fungi-specific primer pairs, and sequenced to analyze the serpentine soils and the non-serpentine soils fungal community.
The pH, EC, and soil water content of serpentine soils were significantly higher than those of non-serpentine soils (P < 0.05). This is likely due to the unique mineral composition of serpentine soils, which are particularly rich in magnesium. During the weathering process, the release of magnesium and increases the alkaline substances in the soil, which raises the pH. Regarding soil water content, it is suggested that the low permeability of serpentine soils contributes to higher moisture retention. The colonization levels of Sasa senanensis were higher in serpentine soils compared to non-serpentine soils, and there was a strong correlation between the levels of colonization and soil pH. This suggests that serpentine soils promote AMF symbiosis. There were significant positive correlations between soil water content and TC or TN, and these values were higher in serpentine soil. Partly humic layer was thick and mixed with soil. That may increase soil water content and organic content. These results indicate that serpentine soils exhibit higher soil water content, TC, and TN compared to non-serpentine soils and more alkaline properties, which combined with higher AMF colonization, suggesting that these factors contribute to the promotion of AMF symbiosis.
The survey was conducted at the Nakagawa and Tesio Experimental Forests of Hokkaido University located in northern Hokkaido. In October 2024, twelve plots were established in adjacent serpentine and non-serpentine rock (sedimentary rock) areas, and the aboveground and belowground parts of Sasa senanensis or Sasa kurilensis grown there, as well as soil up to a depth of 10 cm were collected. Three replicates were taken from each plot. Soil samples were analyzed for pH, electrical conductivity (EC), soil moisture content, total carbon content (TC), and total nitrogen content(TN). Available nitrogen, phosphate, extractive organic nitrogen, extractive organic phosphorus, and heavy metal concentrations (Fe, Zn, Cu, Cr, Ni, Mn, Mg) in the soil were also measured. The collected fine roots were stained, and the colonization levels of AMF were assessed under a microscope. DNA was extracted from both soil and roots, and ITS and 28S rDNA were amplified with the fungi-specific primer pairs, and sequenced to analyze the serpentine soils and the non-serpentine soils fungal community.
The pH, EC, and soil water content of serpentine soils were significantly higher than those of non-serpentine soils (P < 0.05). This is likely due to the unique mineral composition of serpentine soils, which are particularly rich in magnesium. During the weathering process, the release of magnesium and increases the alkaline substances in the soil, which raises the pH. Regarding soil water content, it is suggested that the low permeability of serpentine soils contributes to higher moisture retention. The colonization levels of Sasa senanensis were higher in serpentine soils compared to non-serpentine soils, and there was a strong correlation between the levels of colonization and soil pH. This suggests that serpentine soils promote AMF symbiosis. There were significant positive correlations between soil water content and TC or TN, and these values were higher in serpentine soil. Partly humic layer was thick and mixed with soil. That may increase soil water content and organic content. These results indicate that serpentine soils exhibit higher soil water content, TC, and TN compared to non-serpentine soils and more alkaline properties, which combined with higher AMF colonization, suggesting that these factors contribute to the promotion of AMF symbiosis.