1:45 PM - 3:15 PM
[O11-P21] Coumarine content of Oshima Cherry(Prunus speciosa) in Nishi-izu and Minami-izu
Keywords:Tidal Wind Damage, OOshima Cherry, soil salinity
Introduction
Matsuzaki-cho and Minami-Izu-cho are active producers of cherry leaves. To find out the reason for this, cherry leaves were collected in various Izu areas last year and their coumarin content was measured.
Coumarin is an aromatic component of cherry leaves that is produced as a protective reaction when they are pickled in salt. We hypothesized that the high coumarin content strengthened the aroma of the leaves and led to the development of the cherry leaf industry, and verified whether coumarin content was higher in the Matsuzaki-cho and Minami-Izu-cho areas.
In addition, the survival of the cherry leaf industry is currently in jeopardy due to the aging of the population and the influx of foreign cherry leaves. Through this research, we hope to draw more attention to the cherry leaf industry and help revitalize it.
Materials and Methods
1 Experiments on Injury and Tidal Wind Damage
Using Oshima-zakura cherry trees at the Nirayama Period Theater as samples, cherry leaves were injured with tweezers in the injury experiment and sea water was sprayed onto the leaves with a mist in the tidal wind damage experiment. Each was left uncovered and collected for one week, respectively, and then the procedure for making pickled cherry leaves was followed, and the coumarin content was tested by the Numazu Industrial Technology Center. We also asked Matsuzaki High School to conduct an experiment using the same procedure. In addition, we also asked Niriyama High School to test the coumarin content of the cuttings.
2 Experiments on Soil Salt Damage
Soil samples from five locations collected in the previous year (Jogasaki Beach, Matsuzaki High School, and three cherry blossom fields in Matsuzaki) were divided into beakers and measured using pH and electrical conductivity meters. Soils from Matsuzaki High School and the Nirayama Jidai Theater, which were used in Experiment 1, were also measured.
Results
1 Results of Injury and Tidal Wind Damage Experiments
Coumarin content increased in both injury experiments. In the tidal and wind damage experiments, coumarin content decreased in Nirayama and increased in Matsuzaki. (Table 1) In addition, the coumarin content of the cuttings was significantly lower. (Table 1)
2 Results of the experiment on soil salinity
Regarding electrical conductivity, a correlation was observed between individuals with high coumarin content and the high electrical conductivity of their soils. (Figure 2, Table 2) As for pH, the soils in Sakuragi with high coumarin content were generally neutral. (Figure3, Table 3)
Discussion
1 Discussion from experiments on injury and tidal wind damage
Results indicate that injury to leaves is influenced by coumarin content. Regarding tidal wind damage, an increase in coumarin content was observed at Matsuzaki High School, while the opposite was true at Nirayama High School. The weather conditions during the experiment period were sunny at Matsuzaki High School, while it rained at Nirayama High School, which may have washed away the salt water sprayed on the leaves, reducing the effect of the tidal wind damage. (Table 4)
As for the cuttings, possible reasons for the significantly lower coumarin content could be that the trees were still developing and had been grown in pots. We would like to further study the relationship between the growth condition of the trees and the soil condition of the pots.
2 Discussion from the experiment on soil salinity
The results show that samples with a pH near neutral have dissolved neutral salts, while samples with acidity are more pronounced in the field, suggesting that they are affected by fertilizers, etc. Even in samples with a neutral pH, the high electrical conductivity and the high coumarin content are correlated. The high conductivity correlated with high coumarin content in the pH-neutral samples, suggesting that the amount of neutral salts in the soil may have an effect on the coumarin content. However, since the neutral salt cannot be determined to be sodium chloride in this experiment, we do not know how much salt damage affects the soil.
3 Conclusion
From the experiments conducted in this study, it is possible that coumarin content is influenced by the topography of Matsuzaki.
Comparing the hours of sunlight from March, when the leaves are growing, to July, when they are harvested, at the three sites, we found that in most months,
was longer at Matsuzaki, and focusing on the sea-land wind, it is thought that the wind blows from the western ocean (Table 5). (This wind may carry salts to Matsuzaki (Table 5), which may have an effect on the coumarin content.
Matsuzaki-cho and Minami-Izu-cho are active producers of cherry leaves. To find out the reason for this, cherry leaves were collected in various Izu areas last year and their coumarin content was measured.
Coumarin is an aromatic component of cherry leaves that is produced as a protective reaction when they are pickled in salt. We hypothesized that the high coumarin content strengthened the aroma of the leaves and led to the development of the cherry leaf industry, and verified whether coumarin content was higher in the Matsuzaki-cho and Minami-Izu-cho areas.
In addition, the survival of the cherry leaf industry is currently in jeopardy due to the aging of the population and the influx of foreign cherry leaves. Through this research, we hope to draw more attention to the cherry leaf industry and help revitalize it.
Materials and Methods
1 Experiments on Injury and Tidal Wind Damage
Using Oshima-zakura cherry trees at the Nirayama Period Theater as samples, cherry leaves were injured with tweezers in the injury experiment and sea water was sprayed onto the leaves with a mist in the tidal wind damage experiment. Each was left uncovered and collected for one week, respectively, and then the procedure for making pickled cherry leaves was followed, and the coumarin content was tested by the Numazu Industrial Technology Center. We also asked Matsuzaki High School to conduct an experiment using the same procedure. In addition, we also asked Niriyama High School to test the coumarin content of the cuttings.
2 Experiments on Soil Salt Damage
Soil samples from five locations collected in the previous year (Jogasaki Beach, Matsuzaki High School, and three cherry blossom fields in Matsuzaki) were divided into beakers and measured using pH and electrical conductivity meters. Soils from Matsuzaki High School and the Nirayama Jidai Theater, which were used in Experiment 1, were also measured.
Results
1 Results of Injury and Tidal Wind Damage Experiments
Coumarin content increased in both injury experiments. In the tidal and wind damage experiments, coumarin content decreased in Nirayama and increased in Matsuzaki. (Table 1) In addition, the coumarin content of the cuttings was significantly lower. (Table 1)
2 Results of the experiment on soil salinity
Regarding electrical conductivity, a correlation was observed between individuals with high coumarin content and the high electrical conductivity of their soils. (Figure 2, Table 2) As for pH, the soils in Sakuragi with high coumarin content were generally neutral. (Figure3, Table 3)
Discussion
1 Discussion from experiments on injury and tidal wind damage
Results indicate that injury to leaves is influenced by coumarin content. Regarding tidal wind damage, an increase in coumarin content was observed at Matsuzaki High School, while the opposite was true at Nirayama High School. The weather conditions during the experiment period were sunny at Matsuzaki High School, while it rained at Nirayama High School, which may have washed away the salt water sprayed on the leaves, reducing the effect of the tidal wind damage. (Table 4)
As for the cuttings, possible reasons for the significantly lower coumarin content could be that the trees were still developing and had been grown in pots. We would like to further study the relationship between the growth condition of the trees and the soil condition of the pots.
2 Discussion from the experiment on soil salinity
The results show that samples with a pH near neutral have dissolved neutral salts, while samples with acidity are more pronounced in the field, suggesting that they are affected by fertilizers, etc. Even in samples with a neutral pH, the high electrical conductivity and the high coumarin content are correlated. The high conductivity correlated with high coumarin content in the pH-neutral samples, suggesting that the amount of neutral salts in the soil may have an effect on the coumarin content. However, since the neutral salt cannot be determined to be sodium chloride in this experiment, we do not know how much salt damage affects the soil.
3 Conclusion
From the experiments conducted in this study, it is possible that coumarin content is influenced by the topography of Matsuzaki.
Comparing the hours of sunlight from March, when the leaves are growing, to July, when they are harvested, at the three sites, we found that in most months,
was longer at Matsuzaki, and focusing on the sea-land wind, it is thought that the wind blows from the western ocean (Table 5). (This wind may carry salts to Matsuzaki (Table 5), which may have an effect on the coumarin content.