Japan Geoscience Union Meeting 2025

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[O-11] Senior high school student poster presentations

Sun. May 25, 2025 1:45 PM - 3:15 PM Poster Hall (Exhibition Hall 7&8, Makuhari Messe)

convener:Tatsuhiko Hara(International Institute of Seismology and Earthquake Engineering, Building Research Institute), Keiko Konya(Japan Agency for Marine-Earth Science and Technology), Chieko Suzuki(Japan Agency for Marine-Earth Science and Technology), RYO NAKANISHI(National Institute of Advanced Industrial Science and Technology)


1:45 PM - 3:15 PM

[O11-P88] Evaluation of the Role of Green Fluorescent Protein in Coral Bleaching −Using Aiptasia pulchella-

*Seika Tanida1 (1.KIKAI Institute for Coral Reef Sciences)

Keywords:Coral-reef, Tropical environment, Green fluorescent protein, Biodiversity

Due to global warming, coral bleaching caused by rising seawater temperatures has become increasingly critical. This study investigated the effect of green fluorescent protein (GFP) on bleaching and symbiosis using the model organism Aiptasia pulchella. We evaluated recovery after bleaching, bleaching prevention, and GFP level changes. Methods included fluorescence microscopy analyzed with ImageJ, quantification of GFP fluorescence intensity from extracted samples, dry weight-based analysis, and measurement of photosynthetic activity via oxygen concentration changes. The results suggested that GFP fluorescence intensity increased after bleaching, indicating its potential role in promoting re-symbiosis with zooxanthellae. These findings highlight GFP as a promising factor in developing strategies for coral bleaching mitigation.