9:45 AM - 10:00 AM
[BCG07-04] Cultivation of microbial dark matters from marine sediments: novel bacteria affiliated with uncultured groups of the phylum Chloroflexi
Keywords:Amino acids, Chloroflexi bacteria, Methanogenesis
One of the Chloroflexi bacteria is affiliated with an uncultured group of the class Anaerolineae, and it has been in pure culture (strain MO-CFX2). The strain was isolated from a methanogenic community enriched in a DHS reactor, which was originally obtained from subseafloor sediments off Shimokita Peninsula, Japan. Strain MO-CFX2 grew in anaerobic medium containing yeast extract and peptone. The genome reveals that strain MO-CFX2 may degrade glucose and lactate and also respire halogenated compounds. Currently, we are going to characterize these and other physiological traits in detail.
The other Chloroflexi bacterium belongs to an uncultured lineage of the class Dehalococcoidia, and it is being purified (strain MK-GIF9). This strain was obtained from an anaerobic methane-oxidizing DHS reactor inoculated with biomass collected from Nankai Trough methane seep sediments. The genomic analysis of strain MK-GIF9 indicates that the bacterium can use only several amino acids as energy sources. We also found a glycine fermentation pathway that produces formate as an end product. Based on the genomic analysis, we suspected that the growth of strain MK-GIF9 can be promoted if the metabolic byproduct (formate) is continuously removed by a formate-scavenging partner (e.g., methanogens). Indeed, co-cultivation of MK-GIF9 with a formate-utilizing methanogen successfully stimulated methanogenic glycine degradation and promoted growth of strain MK-GIF9. The specialized ability to cooperatively degrade amino acids sheds light on the poorly understood fate of amino acids in subseafloor sediments. Taken together with the ubiquity and predominance of Chloroflexi bacteria in subseafloor environments, our results implicate that amino acids including glycine may be one of the important precursors for methane production in subseafloor sediments.
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