[BAO01-P12] The diversity and structure determination of saturated and unsaturated archaeol derivatives characteristic for the halophilic archaea lipid-core
Keywords:archaea, isoprenoidal dither, structure determination, halophilic, halite
Then, 1 and 2 were chemically synthesized according to the reported method and the results were presented at the last year’s this meeting. The analysis of the mass fragmentation of the TMS derivative, the structure of microbiological sample derived from halophilc archaea was confirmed as 1. Further, 2 is different from those of Dawson’s unsaturated diether.
About the diversities of these unsymmetrical diether, 1) The isomer of the C25 (long) hydrocarbon is linked with the C-3 of the glycerol 3 was synthesized and mass fragmentation of the TMS ether of 1 and 3 were observed. Teixidor’s report of archaeol derivative from halite were decided to the mixture of 1 and 3 with almost equal amounts. It is suggested that the existence of the unrevealed halophilic archaea which can biosynthesize regioisomeric C25-C20 diether in halite and/or the ancient hypersaline environment. 2) The “real” structure of Dawson’s unsaturated archaeol derivative were assumed to the structure 4 or 5 from the intermediate of biosynthesis of tetraether lipid in thermophilic archaea. Then, the chemical synthesis and mass fragmentation analysis of 4 and 5 will be presented.
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