[EO-11] Stem Cells Application for Dental Implant Osseointegration in Medically Compromised Condition : An In Vivo Study
[Abstract]
[Objective]
Diabetes and osteoporosis, common compromised conditions found in the ageing population, may adversely affect osseointegration of dental implants.1,2 Application of Mesenchymal Stem Cells (MSCs) offer a viable option for this issue.3 This study aimed to evaluate the effect of MSCs to promote implant osseointegration on diabetic and osteoporotic animal models.
[Method]
Forty eight Wistar rats were divided into 4 groups; diabetic control (CD), diabetic+MSCs (TD), osteoporotic control (CO), and osteoporotic+MSCs (TO). Immunohistological (Type 1 Collagen/COL1, Osteocalcin/OCN) and histomorphometric (Bone Implant Contact/BIC) evaluation was done in 2 and 4 weeks then the data was analysed statistically.
[Results and Discussion]
Significant differences were found between treatment and control groups. The highest values of COL1 were found in TD and TO at 2 weeks, indicating MSCs able to increase formation of early stages of bone ossification4 . While for OCN, marker for bone maturation5, MSCs in both diabetic and osteoporotic increased significantly compared to control. MSCs successfully increase BIC value to 67% in 2 weeks and 77% in 4 weeks for diabetic models, while for osteoporotic models, BIC value is 73% in 2 weeks and 79% in 4 weeks. The minimum BIC value for a successful implant is 50% to 80%.4 This study concludes that application of MSCs promotes osseointegration in dental implants on medically compromised conditions.
[References]
1) Tomasiuk JM, Nowakowska-Płaza A, Wisłowska M, et al. Osteoporosis and diabetes - possible links and diagnostic difficulties. Reumatologia. 2023;61(4):294-304.
2) Hendrijantini N, Kuntjoro M, Agustono B, et al. Updating on dental implant osseointegration and survival rate in osteoporotic bone. Indones J Prosthodont. 2022;3(1):53-60.
3) Egusa H, Sonoyama W, Nishimura M, et al. Stem cells in dentistry--Part II: Clinical applications. J Prosthodont Res. 2012;56(4):229-248.
4) Kuntjoro M, Hendrijantini N, Prasetyo EP, et al. "Human umbilical cord mesenchymal stem cells accelerate and increase implant osseointegration in diabetic rats." J Appl Oral Sci. 2023;31:1-13
5) Kamadjaja MJK, Salim S, Rantam FA, et al. Osteogenic Differentiation of Human Amniotic Mesenchymal Stem Cells in Chitosan-Carbonate Apatite Scaffold (In Vivo Study). Contemp Clin Dent. 2018;9(4):592-596.
[Objective]
Diabetes and osteoporosis, common compromised conditions found in the ageing population, may adversely affect osseointegration of dental implants.1,2 Application of Mesenchymal Stem Cells (MSCs) offer a viable option for this issue.3 This study aimed to evaluate the effect of MSCs to promote implant osseointegration on diabetic and osteoporotic animal models.
[Method]
Forty eight Wistar rats were divided into 4 groups; diabetic control (CD), diabetic+MSCs (TD), osteoporotic control (CO), and osteoporotic+MSCs (TO). Immunohistological (Type 1 Collagen/COL1, Osteocalcin/OCN) and histomorphometric (Bone Implant Contact/BIC) evaluation was done in 2 and 4 weeks then the data was analysed statistically.
[Results and Discussion]
Significant differences were found between treatment and control groups. The highest values of COL1 were found in TD and TO at 2 weeks, indicating MSCs able to increase formation of early stages of bone ossification4 . While for OCN, marker for bone maturation5, MSCs in both diabetic and osteoporotic increased significantly compared to control. MSCs successfully increase BIC value to 67% in 2 weeks and 77% in 4 weeks for diabetic models, while for osteoporotic models, BIC value is 73% in 2 weeks and 79% in 4 weeks. The minimum BIC value for a successful implant is 50% to 80%.4 This study concludes that application of MSCs promotes osseointegration in dental implants on medically compromised conditions.
[References]
1) Tomasiuk JM, Nowakowska-Płaza A, Wisłowska M, et al. Osteoporosis and diabetes - possible links and diagnostic difficulties. Reumatologia. 2023;61(4):294-304.
2) Hendrijantini N, Kuntjoro M, Agustono B, et al. Updating on dental implant osseointegration and survival rate in osteoporotic bone. Indones J Prosthodont. 2022;3(1):53-60.
3) Egusa H, Sonoyama W, Nishimura M, et al. Stem cells in dentistry--Part II: Clinical applications. J Prosthodont Res. 2012;56(4):229-248.
4) Kuntjoro M, Hendrijantini N, Prasetyo EP, et al. "Human umbilical cord mesenchymal stem cells accelerate and increase implant osseointegration in diabetic rats." J Appl Oral Sci. 2023;31:1-13
5) Kamadjaja MJK, Salim S, Rantam FA, et al. Osteogenic Differentiation of Human Amniotic Mesenchymal Stem Cells in Chitosan-Carbonate Apatite Scaffold (In Vivo Study). Contemp Clin Dent. 2018;9(4):592-596.