[PPS-O-1] Assessment of cytotoxicity of oil palm based micro crystalline cellulose reinforced acrylic denture base resin.
[Abstract]
Objective
Microcrystalline cellulose (MCC) of the oil palm biomass, with its remarkable reinforcing capability, low density, and environmental benefits, has lately been applied to develop environmentally friendly polymer composites or green composites. It also enhances the mechanical properties of the polymers and has good esthetic attributes1. The aim of this in vitro study was to determine the cytotoxicity of acrylic denture base resin (DBR) material reinforced with oil palm based MCC in different concentrations before and after thermocycling. The cytotoxicity of the conventional and commercially available high impact DBR was also evaluated.
Method
Three different resin mixtures (C, D and E) were prepared from poly (methyl methacrylate) (PMMA) dissolved in methyl methacrylate and mixed with 3 different concentrations of the MCC. The resin C contained an addition of 2% by weight MCC, resin D had 2% MCC replacing equal amount by weight of the PMMA and resin E, 5% MCC replacing equal amount by weight of the PMMA. Resin A used conventional polymer while resin B was commercially available high impact polymer. Extracts were prepared according to ISO 10993-12 from newly made and from thermocycled specimens and their effect on the cell viability of normal human oral fibroblasts (NHOFs) examined by MTT assay. ANOVA (Analysia of Variance) was used to assess any statistically significant differences in cytotoxicity levels among the groups. Results and DiscussionThe results showed that all test groups were non-cytotoxic, with more than 90% viability and no statistically significant difference between the groups. This demonstrates that exposure of NHOFs to eluates from polymer-MCC mixture did not promote cell death or significant toxic effects before and after thermocycling, when compared to the conventional or commercially available high impact DBR material. Correctly processed oil palm based MCC reinforced polymers are not cytotoxic to NHOFs, has the potential to enhance the clinical service life of dentures. and are therefore likely to be safe for use in dental applications.
References
1) Rahaman A, John J, Mani SA, et al: Effect of Thermal Cycling on Flexural Properties of Microcrystalline Cellulose-Reinforced Denture Base Acrylic Resins. J Prosthodont 2020; 29:611-616.
Objective
Microcrystalline cellulose (MCC) of the oil palm biomass, with its remarkable reinforcing capability, low density, and environmental benefits, has lately been applied to develop environmentally friendly polymer composites or green composites. It also enhances the mechanical properties of the polymers and has good esthetic attributes1. The aim of this in vitro study was to determine the cytotoxicity of acrylic denture base resin (DBR) material reinforced with oil palm based MCC in different concentrations before and after thermocycling. The cytotoxicity of the conventional and commercially available high impact DBR was also evaluated.
Method
Three different resin mixtures (C, D and E) were prepared from poly (methyl methacrylate) (PMMA) dissolved in methyl methacrylate and mixed with 3 different concentrations of the MCC. The resin C contained an addition of 2% by weight MCC, resin D had 2% MCC replacing equal amount by weight of the PMMA and resin E, 5% MCC replacing equal amount by weight of the PMMA. Resin A used conventional polymer while resin B was commercially available high impact polymer. Extracts were prepared according to ISO 10993-12 from newly made and from thermocycled specimens and their effect on the cell viability of normal human oral fibroblasts (NHOFs) examined by MTT assay. ANOVA (Analysia of Variance) was used to assess any statistically significant differences in cytotoxicity levels among the groups. Results and DiscussionThe results showed that all test groups were non-cytotoxic, with more than 90% viability and no statistically significant difference between the groups. This demonstrates that exposure of NHOFs to eluates from polymer-MCC mixture did not promote cell death or significant toxic effects before and after thermocycling, when compared to the conventional or commercially available high impact DBR material. Correctly processed oil palm based MCC reinforced polymers are not cytotoxic to NHOFs, has the potential to enhance the clinical service life of dentures. and are therefore likely to be safe for use in dental applications.
References
1) Rahaman A, John J, Mani SA, et al: Effect of Thermal Cycling on Flexural Properties of Microcrystalline Cellulose-Reinforced Denture Base Acrylic Resins. J Prosthodont 2020; 29:611-616.