[Shiau-ROC TAIWAN-O-3] Near-infrared light-enhanced antimicrobial effect of minocycline/hyaluronan/carbon nanohorn conjugate
[Abstract]
[Objective]
With the widespread use of dental implants, peri-implantitis as a complication has arisen, but no effective therapy has been established. An urgent need is to develop effective drug delivery systems (DDS) and apply for peri-implantitis. Carbon nanohorns (CNHs) are highly dispersible and have a large specific surface area that shows promise in drug delivery. We have reported that minocycline (MC) loading on CNHs can impart bacteriostatic activity to CNHs.1 It has also been reported that CNHs can exhibit a photothermal effect, converting light energy into heat when they absorb light. This study exploited the unique properties of CNHs to develop near-infrared (NIR) triggered MC/CNH for peri-implantitis.
[Method]
An aqueous solution of MC/ sodium hyaluronate (HA)/CNH was prepared by mixing aqueous MC with an aqueous solution of a HA/CNH, which CNHs were dispersed in a HA solution. The binding of MC to CNHs was confirmed by absorbance measurement and thermogravimetric analysis (TGA). The dispersion stability of MC/HA/CNH was demonstrated. To confirm bacteriostatic properties, MC/HA/CNH solution was added to the bacterial suspension and the number of colonies forming units (CFU) was compared. Furthermore, the bacteriostatic properties were also compared after dialysis of the MC/HA/CNH solution for 48 hours. CFU was analyzed by a one-way ANOVA test and Uncorrected Dunn's test.
[Results and Discussion]
Bacterial culture experiments revealed the bactericidal effect of MC/HA/CNH increased under NIR light irradiation. It was also shown that the effect persisted even after 48 hours of dialysis. These results suggest that the bactericidal effect of MC/CNH was enhanced by the photothermal effect of CNH and that of MC/CNH continues at least for 48hrs. MC/HA/CNH is more effective with lower concentrations compared to the conventional administration of MC and a promising new treatment strategy for peri-implantitis.
[References]
1) Maeda Y, Hirata E, Takano Y, et al. Carbon, 2020, 166, 36–45.
[Objective]
With the widespread use of dental implants, peri-implantitis as a complication has arisen, but no effective therapy has been established. An urgent need is to develop effective drug delivery systems (DDS) and apply for peri-implantitis. Carbon nanohorns (CNHs) are highly dispersible and have a large specific surface area that shows promise in drug delivery. We have reported that minocycline (MC) loading on CNHs can impart bacteriostatic activity to CNHs.1 It has also been reported that CNHs can exhibit a photothermal effect, converting light energy into heat when they absorb light. This study exploited the unique properties of CNHs to develop near-infrared (NIR) triggered MC/CNH for peri-implantitis.
[Method]
An aqueous solution of MC/ sodium hyaluronate (HA)/CNH was prepared by mixing aqueous MC with an aqueous solution of a HA/CNH, which CNHs were dispersed in a HA solution. The binding of MC to CNHs was confirmed by absorbance measurement and thermogravimetric analysis (TGA). The dispersion stability of MC/HA/CNH was demonstrated. To confirm bacteriostatic properties, MC/HA/CNH solution was added to the bacterial suspension and the number of colonies forming units (CFU) was compared. Furthermore, the bacteriostatic properties were also compared after dialysis of the MC/HA/CNH solution for 48 hours. CFU was analyzed by a one-way ANOVA test and Uncorrected Dunn's test.
[Results and Discussion]
Bacterial culture experiments revealed the bactericidal effect of MC/HA/CNH increased under NIR light irradiation. It was also shown that the effect persisted even after 48 hours of dialysis. These results suggest that the bactericidal effect of MC/CNH was enhanced by the photothermal effect of CNH and that of MC/CNH continues at least for 48hrs. MC/HA/CNH is more effective with lower concentrations compared to the conventional administration of MC and a promising new treatment strategy for peri-implantitis.
[References]
1) Maeda Y, Hirata E, Takano Y, et al. Carbon, 2020, 166, 36–45.