[P3-0807] Reinnervation ability on healing process of anterior cruciate ligament injury in adult rats
キーワード：reiinervation, ACL injury, growth associated protein 43
【Purpose】
One of the most common knee injuries is an anterior cruciate ligament injury. Once ACL damaged, the ligament has poor healing ability. The results leads to the instability, loss of motor and sensory functions of the knee joints. The purpose of this study was to evaluate the gene expression with neurotrophin and nerve element during the process of ACL healing.
【Methods】
Adult Wistar male rats（10 weeks old）were randomly assigned to three groups, ACL transection（ACL-T；n=5）, ACL transection and surgery of joint stability（JC：n=5）, Sham op（Sham；n=5）. ACLs were harvested at 2 and 4 weeks after surgery. Quantitative analysis for Brain-derived neurotrophic factor（BDNF）, growth associated protein 43（GAP43）were performed using realtime PCR. These mRNA expression were normalized respect to internal house keeping gene（GAPDH）using ΔΔCT method Statistical analysis was undertaken using the one way ANOVA with the Tukey post hoc test.
【Results】
The expression of BDNF mRNA was 2.7 fold higher in JC than sham JC. ACL-T was 0.5 fold higher than sham. But there is no significant change in among group. GAP43 mRNA was 174 fold higher in JC than sham（P＜0.01）, ACL-T was 54 fold higher than sham（P＜0.01）.
【Discussion】
We observed that joint reaction caused by stability read to heal the injured ACL. In the healing process of the ligament, Injuries to knee is associated with a decline in neuromuscular and somatosensory functions, whereas our findings indicate that increases expression of neurotrophins and reinnervation in the ACL injury. Normal movement of the knee joint alters in biomechanical microenvironment changes of the joint, providing BDNF and GAP43 by physiological process that it is endogenous in the ligament. This findings might have important consequences for neuroal plasticity and regeneration in the neuromuscular system in the knee.
One of the most common knee injuries is an anterior cruciate ligament injury. Once ACL damaged, the ligament has poor healing ability. The results leads to the instability, loss of motor and sensory functions of the knee joints. The purpose of this study was to evaluate the gene expression with neurotrophin and nerve element during the process of ACL healing.
【Methods】
Adult Wistar male rats（10 weeks old）were randomly assigned to three groups, ACL transection（ACL-T；n=5）, ACL transection and surgery of joint stability（JC：n=5）, Sham op（Sham；n=5）. ACLs were harvested at 2 and 4 weeks after surgery. Quantitative analysis for Brain-derived neurotrophic factor（BDNF）, growth associated protein 43（GAP43）were performed using realtime PCR. These mRNA expression were normalized respect to internal house keeping gene（GAPDH）using ΔΔCT method Statistical analysis was undertaken using the one way ANOVA with the Tukey post hoc test.
【Results】
The expression of BDNF mRNA was 2.7 fold higher in JC than sham JC. ACL-T was 0.5 fold higher than sham. But there is no significant change in among group. GAP43 mRNA was 174 fold higher in JC than sham（P＜0.01）, ACL-T was 54 fold higher than sham（P＜0.01）.
【Discussion】
We observed that joint reaction caused by stability read to heal the injured ACL. In the healing process of the ligament, Injuries to knee is associated with a decline in neuromuscular and somatosensory functions, whereas our findings indicate that increases expression of neurotrophins and reinnervation in the ACL injury. Normal movement of the knee joint alters in biomechanical microenvironment changes of the joint, providing BDNF and GAP43 by physiological process that it is endogenous in the ligament. This findings might have important consequences for neuroal plasticity and regeneration in the neuromuscular system in the knee.