[13p-PB7-1~14] English session: Asian Joint Symposium on Nanobiotechnology

▲ [13p-PB7-7] Rapid MRSA gene detection device for bedside monitoring

〇kazuya takahashi¹, Saito Masato¹, Yukihiro Akeda², Kazunori Tomono³, Eiichi Tamiya¹ (1.Osaka Univ.Grad.Eng., 2.Osaka Univ.RIMD, 3.Osaka Univ. Grad. Med.)

キーワード：POCT,Rapid PCR

Avoiding opportunistic infection caused by drug resistant bacteria (i.e., MRSA (Methicillin-resistant Staphylococcus aureus)) is crucial especially for weak people in the hospital. MRSA has mecA gene that is the cause of its drug resistance. PCR based genetic test is a gold standard for gene detection and identification. POCT (Point-of-Care Testing) PCR device can be utilized for detection of various inspection targets in many cases such as medicine and food safety. A variety of PCR methods, for example conventional PCR using thermal cycler, flow PCR and convective PCR utilizing thermal convection(SCIENCE 298, 793, 2002), have previously been developed. However, these conventional PCR methods require complicated sample preparations, trained users and large apparatus for on-site inspection. Accordingly, PCR system had been developed in our laboratory, which applied thermal convection and centrifugal force, aimed at expediting and simplifying PCR process and downsizing the device. An automated dispensing system had been previously developed, using capillary and centrifugal force to dispense specified amounts of reagents into the micro channel. With these techniques, PCR can be conducted without well-trained users and large apparatus and be applied to on-site diagnosis.
In this report, optimization of MRSA genomic DNA detection was achieved and calibration curve using for various concentration of MRSA genomic DNA was derived. The device has been improved for multi-targeting and bedside inspection. The improved device can be tested for rapid and simultaneous detection of drug resistant bacteria like MRSA.