[14a-PB1-1~10] 3.4 Biomedical optics

[14a-PB1-6] Two-photon fluorescence microscopy by combining temporal focusing with 3D structured illumination

〇Keisuke Isobe^1,2, Qiyuan Song^1,3, Keisuke Toda^1,4, Fumihiko Kannari³, Hiroyuki Kawano¹, Atsushi Miyawaki¹, Katsumi Midorikawa¹ (1.RIKEN, 2.JST-PRESTO, 3.Keio Univ., 4.Saitama Univ.)

Keywords:multiphoton fluoreascence microscopy,super-resolution imaging

Multiphoton fluorescence microscopy using the temporal focusing technique is a powerful tool for obtaining a cross-sectional image without scanning the laser beam. In this presentation, we show that the use of combination of temporal focusing and 3D structured illumination provides the enhancement of 3D spatial resolution.