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[14p-2N-13] In-situ observation system for pulsatile flow velocity field using CS-MLDV
Keywords:laser,blood flow,multi point
We have described the cross sectional multipoint LDV (CS-MLDV) system for monitoring blood vessels which are sutured and connected during an operation. To observe condition of a blood vessel during an operation, the blood flow diagnostic equipment requires in-situ imaging of the blood flow velocity distribution with both high-spatial and high-time resolution. Therefore, we developed CS-MLDV, which is improvement of conventional LDV system. In-vivo blood flow measurements have been recently demonstrated and have been used in the diagnosis using LDV. In the case of the conventional LDV, 2D imaging can be performed by moving the measurement point. Hence, the measurements are not simultaneous for all points within the 2D region.
The CS-MLDV can measure the instantaneous two-dimensional (2D) flow velocity by upgrading the optical component and data processing from conventional LDV. In particular, for the measuring of 2D velocity distribution, the 2D optical fiber array was developed. In this approach, the intersection area of the two laser light sheets forms a 2D measurement region. Laser Doppler signals are emitted from different points in this region, and these are detected using the 2D optical fiber array. This array consists of 64 optical fibers arranged in an 8 x 8 grid pattern. Each optical fiber is 0.23 mm in diameter and the core is 0.19 mm in diameter. The scattered light is transmitted through the each optical fiber and detected by a silicon avalanche photodiodes (Si-APDs). Absolute velocity is estimated from Doppler signals.
In this study, we carried out in-vivo blood flow measurements in a mesenteric vessel of a mouse using CS-MLDV. It means that the in-situ blood flow imaging was performed. Scanning was performed in the measurement region in the mesentery. Therefore, CS-MLDV system has a possibility of being used effectively in the operation. For example, whether the blood normally flows or not in the sutured blood vessel can be observed by using CS-MLDV. We could observe flow velocity of blood flow by using CS-MLDV system. The CS-MLDV can be applied to the diagnose equipment during an angiography operation due to have the high temporal-spatial resolution.
The CS-MLDV can measure the instantaneous two-dimensional (2D) flow velocity by upgrading the optical component and data processing from conventional LDV. In particular, for the measuring of 2D velocity distribution, the 2D optical fiber array was developed. In this approach, the intersection area of the two laser light sheets forms a 2D measurement region. Laser Doppler signals are emitted from different points in this region, and these are detected using the 2D optical fiber array. This array consists of 64 optical fibers arranged in an 8 x 8 grid pattern. Each optical fiber is 0.23 mm in diameter and the core is 0.19 mm in diameter. The scattered light is transmitted through the each optical fiber and detected by a silicon avalanche photodiodes (Si-APDs). Absolute velocity is estimated from Doppler signals.
In this study, we carried out in-vivo blood flow measurements in a mesenteric vessel of a mouse using CS-MLDV. It means that the in-situ blood flow imaging was performed. Scanning was performed in the measurement region in the mesentery. Therefore, CS-MLDV system has a possibility of being used effectively in the operation. For example, whether the blood normally flows or not in the sutured blood vessel can be observed by using CS-MLDV. We could observe flow velocity of blood flow by using CS-MLDV system. The CS-MLDV can be applied to the diagnose equipment during an angiography operation due to have the high temporal-spatial resolution.