[14p-2N-1~16] 3.4 Biomedical optics

▲ [14p-2N-2] Two-photon microscopy with an 1μm-band wavelength-selectable picosecond light pulse source based on a gain-switched laser diode

〇YICHENG FANG¹, YUTA KUSAMA¹, RYOSUKE KAWAKAMI², TOMOMI NEMOTO², HIROYUKI YOKOYAMA¹ (1.NICHe, Tohoku Univ., 2.RIES, Hokkaido Univ.)

Keywords:two photon microscopy,supercontinuum light,gain switched laser diode

Two-photon fluorescent microscopy (TPM) has had profound implications for investigations of complex biological samples. For TPM, the development of a compact, highly functional optical pulse source is thus highly desirable. We have developed a high-peak-power picosecond light pulse source based on a 1060nm-band gain-switched laser diode (GSLD). This enabled deep-site observations of neuron cells of a mouse brain expressing enhanced yellow fluorescent proteins (eYFP). Although our previous 1060nm-band light pulse source was a wavelength-fixed one, a wavelength tunable (selectable) feature is desired to efficiently excite fluorescent materials. We here report the results for extending the light pulse source feature to wavelength-tunable around 1μm band and for utilizing it to demonstrate the wavelength-dependence of two-photon imaging of neuron cells expressing eYFP.