13:15 〜 13:45
▲ [14p-3A-1] Aptamer Nanobiosensors for Ultrasensitive Detection of Pandemic viruses, Type 2 Diabete Biomarkers, and Antibiotics
キーワード：Aptamers,Grephene Oxide,Sandwich Assay
Aptamers are single-stranded nucleic acids having molecular recognition properties similar to antibodies, and isolated by in vitro selection and amplification process, SELEX. Aptamer engineering includes innovative screening and modification of aptamers for maximizing their function. This talk will start with how the aptamers are innovatively screened, for the first time in world, by using a new nano-material, graphene oxide, without needing the immobilization of targets. A few of successful examples for a couple of pandemic viruses and diabete type 2 biomarkers will be presented based on the aptamer duo screened by GO-SELEX, including a noble aptamer-duo story, i.e. inevitable in a sandwich platform. Once the aptamers are successfully screened out, the aptamers can be further engineered, by truncation. In this talk, it will be presented how the aptamer candidates are analyzed and heuristically engineered for developing the world shortest aptamer, octamer, with highly enhanced affinity. This sequence-based engineering of aptamers has resulted in a very successful ultrasensitive biosensing, especially for the detection of antibiotics, which can never been able to be reached without engineering of aptamers. In addition, the benefits of using nano-sized materials for biosensing will be presented with scientifically proven clear examples of different hybrid forms of nanomaterials and aptamers, such as aptamers-gold nanoparticle composites, aptamers-in-liposome, and aptamers-on-nanofibre.
[1] J.-W. Park, R. Tatavarty, D.W. Kim, H.-T. Jung and M.B. Gu (2012) Immobilization-free screening of aptamers assisted by graphene oxide, Chem. Comm., 48,15, 2071-2073
[2] S. J. Lee, Y.S. Kwon, J. Lee, E.-J. Choi, C.-H. Lee, J.-Y. Song and M.B. Gu (2013) Detection of VR-2332 strain of Porcine Reproductive and Respiratory and Syndrome Virus Type II by Using an Aptamer-based Sandwich-Type Assay, Anal. Chem., 85, 66-74
[3] Y. S. Kwon, N. H. Ahmad Raston and M.B. Gu (2014) An Ultra-sensitive Colorimetric Detection of Tetracyclines using the Shortest Aptamer with Highly Enhanced Affinity, Chem. Comm., 50, 1, 40-42
[4] J.-W. Park, S. J. Lee, and M.B. Gu (2014) An Ultra-sensitive Detection of a Whole Virus using Dual Aptamers developed by Immobilization-free Screening, Biosensors and Bioelectronics, 51, 324–329
[5] Van Thuan Nguyen, Young Seop Kwon, JaeHoon Kim, and M.B. Gu (2014) Multiple GO-SELEX for Efficient Screening of Flexible Aptamers, Chem. Comm., 139, 18, 4696-4701
[6] N.H. Ahmad Raston and M.B. Gu (2015) Highly amplified detection of visceral adipose tissue-derived serpin (vaspin) using a cognate aptamer duo, Biosensors and Bioelectronics, 70, 261-267.
[1] J.-W. Park, R. Tatavarty, D.W. Kim, H.-T. Jung and M.B. Gu (2012) Immobilization-free screening of aptamers assisted by graphene oxide, Chem. Comm., 48,15, 2071-2073
[2] S. J. Lee, Y.S. Kwon, J. Lee, E.-J. Choi, C.-H. Lee, J.-Y. Song and M.B. Gu (2013) Detection of VR-2332 strain of Porcine Reproductive and Respiratory and Syndrome Virus Type II by Using an Aptamer-based Sandwich-Type Assay, Anal. Chem., 85, 66-74
[3] Y. S. Kwon, N. H. Ahmad Raston and M.B. Gu (2014) An Ultra-sensitive Colorimetric Detection of Tetracyclines using the Shortest Aptamer with Highly Enhanced Affinity, Chem. Comm., 50, 1, 40-42
[4] J.-W. Park, S. J. Lee, and M.B. Gu (2014) An Ultra-sensitive Detection of a Whole Virus using Dual Aptamers developed by Immobilization-free Screening, Biosensors and Bioelectronics, 51, 324–329
[5] Van Thuan Nguyen, Young Seop Kwon, JaeHoon Kim, and M.B. Gu (2014) Multiple GO-SELEX for Efficient Screening of Flexible Aptamers, Chem. Comm., 139, 18, 4696-4701
[6] N.H. Ahmad Raston and M.B. Gu (2015) Highly amplified detection of visceral adipose tissue-derived serpin (vaspin) using a cognate aptamer duo, Biosensors and Bioelectronics, 70, 261-267.