18:15 〜 18:30
▼ [14p-3A-12] Fully automated Microelectrofluidic device for diagnostic application
キーワード：microfluidic,biosensing
We propose a fully automated microelectrofluidic device with integrated fluidic channels, sensing and novel thin film reference electrodes and detection circuitry. The developed device is able to operate standalone from sample in to detection with the help of software controlled fluidic valves which means no manual operation is required. The proposed device is expected to be a milestone for future on chip disease diagnosis such as early cancer detection.
For decades, molecular biosensing had to rely on one of the highly accurate but expensive and time consuming method of detection – fluorescence based detection that requires the attachment of label to the target molecules. There have been alternative attempts such as CMOS based devices [1], SiNW field effect based biosensor [2] etc. all presenting different techniques for electrical detection of different nucleic acids. Reported works have shown the ability of electrical detection methods in smaller molecule detection. But rapid, highly specific, on-chip disease diagnostic is still a distant thought. This is because the development of such kind of platform requires assistance from multiple technology and integration of different technologies bring new challenges to meet.
We recently demonstrated [3] palm sized microfluidic platform integrated with analog circuit for label free, portable, and low volume sensing. We then attempted [4] exploring our work on the detection and separation of miRNAs and investigated smaller but significant molecular binding effects such as coaxial stacking effect resulted in a sandwich hybridization technique to improve sensitivity and selectivity. Here, we focused on a device design to achieve the rapid detection and portability. The device (Fig.1) contains microfluidic channels and valves controllable by software for sample circulation, mixing, washing etc., a sensing surface, a novel highly stable thin film micro reference electrode to increase portability and inerrability, and an on chip miniature analog circuit for the detection. The device is expected to be a great step toward future point of care early cancer detection.
For decades, molecular biosensing had to rely on one of the highly accurate but expensive and time consuming method of detection – fluorescence based detection that requires the attachment of label to the target molecules. There have been alternative attempts such as CMOS based devices [1], SiNW field effect based biosensor [2] etc. all presenting different techniques for electrical detection of different nucleic acids. Reported works have shown the ability of electrical detection methods in smaller molecule detection. But rapid, highly specific, on-chip disease diagnostic is still a distant thought. This is because the development of such kind of platform requires assistance from multiple technology and integration of different technologies bring new challenges to meet.
We recently demonstrated [3] palm sized microfluidic platform integrated with analog circuit for label free, portable, and low volume sensing. We then attempted [4] exploring our work on the detection and separation of miRNAs and investigated smaller but significant molecular binding effects such as coaxial stacking effect resulted in a sandwich hybridization technique to improve sensitivity and selectivity. Here, we focused on a device design to achieve the rapid detection and portability. The device (Fig.1) contains microfluidic channels and valves controllable by software for sample circulation, mixing, washing etc., a sensing surface, a novel highly stable thin film micro reference electrode to increase portability and inerrability, and an on chip miniature analog circuit for the detection. The device is expected to be a great step toward future point of care early cancer detection.