[14p-3A-1~12] English session: Asian Joint Symposium on Nanobiotechnology

▲ [14p-3A-4] Application of Nanoplasmonics-based Microfluidic Sensor for Highly Sensitive Biomedical Detection

〇Jaebum Choo¹, Joonki Hwang¹, Jimin Yu¹, Jinhyeok Jeon¹ (1.Hanyang University)

Keywords:Nano-Plasmonics,Microfluidics,Immunoassay

Metal nanoparticle is emerging as one of the most effective optical detection tools in nanobiotechnology. Depending on their chemical composition, their optical properties have garnered much attention in biological studies approaching molecular dimensions. In this presentation, we report a quick and reproducible nanoplasmonics-based immunoassay technique using hollow gold nanospheres (HGNs) and magnetic beads. HGNs show strong SERS enhancement effects from individual particles because hot spots can be localized within pinholes in the hollow particle structure. Accordingly, HGNs can be used for highly reproducible immunoanalysis of cancer markers. Magnetic beads were used as supporting substrates for the formation of the immunocomplex. In addition, a microfluidic platform allows the integration of magnetic bars for low-volume immunoassays. A nanoplasmonics-based sandwich immunoassay has been performed on chip in an automatic manner. For validation, well-known cardiac markers (troponin I and CK-MB) were used as targets. Based on experimental results, the limit of detection (LOD) was determined to be 1–10 pg/mL, this value being about 100–1000 times more sensitive than the LOD of conventional enzyme-linked immunosorbent assay (ELISA). This technique is expected to be a powerful clinical tool for fast and reliable cancer diagnosis. In this presentation, a novel nanoplasmonics-based microfluidic sensing technique for rapid and sensitive immunoassay will be introduced.