2:30 PM - 2:45 PM
[15p-2A-4] Deceleration of single-stranded DNA passing through a nanopore using a nanometre-sized bead structure
Keywords:nanopore,DNA sequencer,nanoparticle
Nanopore DNA sequencing is undergoing rapid development for its potential advantages (i.e., long reads, and low cost) compared with existing DNA sequencing methods. Based on its composition, nanopore-related technologies are categorized as either a biological nanopore technology or a solid-state nanopore technology. Although significant advancements have been achieved for biological nanopore, solid-state nanopores are more promising because of their higher robustness and stability of material. One of the primary issues in actualizing the solid-state nanopore sequencing, however, is that the DNA translocation through the nanopore is too rapid relative to the sampling rate of the ammeter. In this study, we report that a nanometre-sized bead structure constructed around a nanopore can reduce the moving speed of single-stranded DNA (ssDNA) to 270 μs/base by adjusting the diameter of the bead and its surface chemical group. This decelerating effect originates from the strong interaction between ssDNA and the chemical group on the surface of the bead. This nanostructure was simply prepared by dip coating in which a substrate with a nanopore was immersed in a silica bead solution and then dried in an oven. As compared with conventional approaches, our novel method is less laborious, simpler to perform and more effective in reducing ssDNA translocation speed.