[15p-2B-1~16] 12.7 Biomedical Engineering and Biochips

△ [15p-2B-10] Detection of Norovirus Virus-Like Particles Using a V-Trench Biosensor

〇Yuki Sugiyama¹, Chiaki Kuroda¹, Yoshimichi Ohki^1,2, Hiroki Ashiba³, Xiaomin Wang³, Makoto Fujimaki³ (1.GSASE, Waseda Univ., 2.RIMST, Waseda Univ., 3.AIST)

Keywords:enhanced fluorescence,surface plasmon resonance,norovirus

Surface plasmon (SP) excitation provides enhancement of fluorescence intensity and better sensitivity than conventional fluorescent immunoassay. However, the SP excitation requires optimal positioning of a prism to ensure optimum output of the incident light. We have developed a "V-trench biosensors" combining the V-shaped fluidic channels for detection. The V-trench biosensor can use the fluorescence enhancement caused by surface plasmon resonance excitation easily and at low cost. Recently, we proposed the use of quantum dots as a fluorescent label for the V-trench biosensor. Since the quantum dots have a large Stokes shift, we can effectively remove the exciting light and reduce background signals. In this study, we developed a V-trench sensor chip for the quantum dot fluorescent labels. Moreover, we attempted to detect the norovirus virus-like particles using the developed sensor chips.