[16a-2C-1~9] 4.2 Bio- and Medical Photonics

▲ [16a-2C-1] [JSAP-OSA Joint Symposia 2015 Invited Talk] Super-resolution optical fluctuation imaging with high spatiotemporal resolution

Xuanze Chen¹, Zhiping Zeng¹, 〇Peng Xi¹ (1.Peking University)

Keywords:super-resolution,microscopy,SOFI

Super-resolution microscopy opens up a new era for visualizing the dynamic subcellular structure and interaction in a whole new scale. The methods to achieve super-resolution can be largely divided into two categories: (1) targeted modulation; and (2) stochastic modulation/fluctuation analysis. In stochastic single-molecule localization microscopy, the spatial resolution is gained through the scarification of temporal resolution, to ensure the sufficient sampling of the specimen. However, in super-resolution microscopy, the high spatial resolution is generally at a price of the imaging speed. Super-resolution optical fluctuation imaging (SOFI) is a new technique which can allow multiple emitters within one diffraction limited region to be at on state simultaneously, and using the fluctuation dynamics to achieve super-resolution. Thus, it provides high spatial and temporal resolution simultaneously. Here we report several advances of SOFI: (1) to enable high spatiotemporal resolution of SOFI (via JT-SOFI); (2) better 3D imaging capability (via 3D-MUSIC); and (3) improved in vivo imaging capability with Skylan-S.