[16a-2V-1~13] 8.6 Plasma life sciences

△ [16a-2V-6] Induction of Plant Defense Gene Expression by Atmospheric Air Plasma in Oryza Sativa

〇Hideaki Koniahi¹, Keisuke Takashima¹, Sugihiro Ando², Hideki Takahashi², Toshiro Kaneko¹ (1.Dept. of Electronic Eng., Tohoku Univ., 2.Dept. of Life Sci., Grad. Sch. of Agricultural Sci., Tohoku Univ.)

Keywords:Plasma Agriculture,Defense Related Genes,Reactive Oxigen Species

We report induction effect on defense genes of Oryza sativa by atmospheric pressure plasma. A plasma jet using air and water has been developed. Germinated Seeds of Oryza sativa are irradiated by the plasma. They are grown under dark at 28℃ for 2, 5, or 8 days. Total RNA is isolated from plants, and reverse transcribed into cDNA for quantitative RT-PCR amplification detecting Os-PBZ1 and Os-POX. The genes are expressed by the plasma. Further experimentation is undergoing.