The 76th JSAP Autumn Meeting, 2015

Presentation information

Oral presentation

4 JSAP-OSA Joint Symposia 2015 » 4.2 Bio- and Medical Photonics

[16p-2C-1~10] 4.2 Bio- and Medical Photonics

Wed. Sep 16, 2015 1:45 PM - 5:00 PM 2C (212-1)

Chair:Yuji Matsuura(Tohoku Univ.)

3:00 PM - 3:15 PM

[16p-2C-5] SERS immunosensor for detecting influenza A

〇(D)Kullavadee Karnorachai1,2, Kenji Sakamoto1, Rawiwan Laocharoensuk3, Suwussa Bamrungsap3, Tararaj Dharakul3,4, Kazushi Miki1 (1.NIMS, Tsukuba, Japan, 2.Tsukuba Univ., 3.NANOTEC, NSTDA, 4.Mahidol Univ.)

Keywords:Biosensor,SERS

Surface-enhanced Raman spectroscopy (SERS) has great promise as a technique for detecting trace amounts of biological and chemical samples, because of its advantages, e.g. nondestructive, no photobleaching, sensitive and fast detection. Our SERS immunosensor is composed of a Au@Ag two-dimensional (2D) array substrate and SERS probes, which are 4, 4’-thiobisbenzenethiol (TBBT)-labeled AuNPs conjugated with antibodies. The detection of influenza A nucleoproteins is achieved by observing the Raman signal of TBBT. We have succeeded in selective detection of influenza A nucleoproteins. We found that the detection sensitivity is increased by using Au@Ag2D array substrate, instead of a SERS inactive substrate (Au film). This improvement is attributed to the plasmonic coupling between the SERS substrate and SERS probes.