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△ [14a-B10-2] Static Charge outside Chamber Induces Dielectric Breakdown to Solid-state Nanopore Membranes
Keywords:Nanopore, Dielectric breakdown, Static charge
In order to realize DNA sequencing with a low noise level using a solid-state nanopore, the capacitance of the device has to be reduced. However, we recently found that pinhole-like defects were generated when the low capacitance devices were operating. The defects turned out to be generated by dielectric breakdown due to an unexpected high voltage between the electrolytes. This is a serious problem with the low capacitance devices, but the main origin of the voltage had not been clarified.
In this research, we elucidated that the voltage was generated mainly due to static charge on the surface of flow cells outside the chambers. The surface voltage on the flow cells is divided to the membrane according to capacitance coupling ratio of the flow cell and the membrane. We also showed that the generation of the defects was prevented using new flow cells coated with anti-static agent.
In this research, we elucidated that the voltage was generated mainly due to static charge on the surface of flow cells outside the chambers. The surface voltage on the flow cells is divided to the membrane according to capacitance coupling ratio of the flow cell and the membrane. We also showed that the generation of the defects was prevented using new flow cells coated with anti-static agent.