[14a-C301-1~9] 4.5 Information Photonics

▲ [14a-C301-3] Multi-modal Digital Holographic Microscope with Dual-wavelength Excitation and Dual-wavelength Phase Imaging

〇(D)Xiangyu Quan¹, Osamu Matoba¹, Kouichi Nitta¹, Yasuhiro Awatsuji², Yukako Takizawa³ (1.Kobe Univ., 2.Kyoto Inst. Tech., 3.Hyogo Pref. Inst. Tech.)

Keywords:Digital holography, Multi-modal

This paper presents utilizing multi wavelengths of fluorescent excitation and hologram recording. This is realized by using a pair of 473 nm and 532 nm lasers as excitation light, and another pair of 632.8 nm and 830 nm as hologram formation. Using two or more kinds of excitation light would increase the number of visible colors, thus multiple marking would be possible. On the other hand, measured phase value is limited to range of [-pi,pi] without special phase unwrapping treatment. Using different wavelengths will solve this problem by increasing measurable range. Experiments using microbeads and plant cells are demonstrated.