Keywords:self-interference digital holography, fluorescence nanoparticle, optical tweezers
Three-dimensional mapping of fluorescence light sources was performed by using a self-interference digital holography. The positions of the sources were quantitatively determined by using the Gaussian fitting of the axial and lateral diffraction distributions through position calibration from the observation space to the sample space. The space division matching method was developed to perform the mapping of dense fluorescence sources. The mapping of fluorescence nanoparticles fixed in gelatin and a single fluorescent nanoparticle manipulated with optical tweezers in water were demonstrated.