[14p-C301-1~9] 4.3 Optical Micro-sensing, Manipulation, and Fabrications

▲ [14p-C301-5] [JSAP-OSA Joint Symposia 2016 Invited Talk] DNA Sensing Based on Gold Nanoparticles and Silicon Nanopores

〇Toshiharu Saiki¹, Hirohito Yamazaki¹, Takaha Mizuguchi¹, Keiko Esashika¹ (1.Keio Univ.)

Keywords:Nano Optics, Nano Fluidics, Phase Change Material

A robust method to distinguish isolated single gold nanoparticles (AuNP monomers) and their dimers under Brownian motion is described as a key technique for ultrasensitive homogeneous bioassays, including AuNP sandwich assays. We also propose a new optical measurement scheme for nanopore-based DNA detection and sequencing. An optical microscopic measurement with 100-nm and 100-μs resolution is demonstrated to observe DNA coiling immediately after translocation and its motion. The effect of salt concentration and counter-ion species on the translocation speed and dwell time in the observation volume is also investigated.