[14p-P23-1~11] English session: Joint symposium on Nanobiotechnology and Biosensing

▲ [14p-P23-2] Sensitivity Enhancement of Electrochemical-Surface Plasmon Resonance IgG Immunosensor Based on Graphene Oxide/Poly(2-Aminibensylamine) Film

〇(D)Chammari Pothipor^1,2, Kontad Ounnunkad², Chutiparn Lertvachirapaiboon¹, Kazunari Shinbo¹, Keizo Kato¹, Futao Kaneko¹, Akira Baba¹ (1.Niigata Univ. for Niigata University, 2.Chiang Mai Univ. for Chiang Mai University)

Keywords:electrochemical surface plasmon resonance, graphene oxide, poly(2-aminobenzylamine)

An electrochemical surface plasmon resonance immunosensor (EC-SPR) is a technique for estimation of biomolecular interaction in real-time. In this research, The EC-SPR biosensor based on poly(2-aminobenzylamine)/graphene oxide (P2ABA/GPO) was developed for the detection of human IgG. Figure 1A represents EC-SPR setup. GPO dispersed in 2ABA monomer solution was deposited on gold surface by cyclic voltammetry with a potential range of -0.2 to 1.1 V for 2 cycles at a scan rate of 20 mV/s. After electropolymerization of the P2ABA, the SPR dip shift was observed and then antibodies were covalently bound onto the platform. At the same condition, a film obtained from GPO-2ABA solution showed higher current response due to better electroactivity. The observable enhancement of IgG detection on P2ABA/GPO (solid line) compared with that on P2ABA (dash line) is shown in Figure 1B. The concentration dependence exhibited a linear relationship with human IgG in the range from 0.5 to 10 µg/mL and the sensitivity of 0.011 µg/mL with the lowest detection limit of 0.135 µg/mL, suggesting good device performances in the detection of human IgG is shown in Figure 1C and 1D.