9:45 AM - 10:15 AM
▲ [15a-C31-3] [JSAP-OSA Joint Symposia 2016 Invited Talk] Hybrid light microscopy methods for multi-dimensional imaging of small animal models
Keywords:Light microscopy, Imaging speed, Optical sectioning
Light microscopy methods have been widely used in biomedical research. Different microscopy techniques have different advantages and disadvantages. For example, wide-field optical microscopy is known for its convenience and high imaging speed. However, it has no depth sectioning capability due to the missing axial high-frequency components in its 3D optical transfer function. It is therefore impossible to obtain 3D structures of specimen based on wide-field images stacks. Laser scanning microscopes (e.g., confocal microscopes), on the other hand, can provide the so-called optical sectioning capability and high axial resolution. While conventional confocal microscopy is a standard tool for 3D visualization of fixed samples, their slow scanning speed and excessive photobleaching make it less suitable for real-time in vivo imaging of live samples. We have developed a hybrid strategy to combine information information from both scanning microscopy and wide-field microscopy imaging modes. A prototype hybrid microscope has been designed and implemented. We have verified its imaging performances, such as reduced photobleaching, improved imaging speed, and improved optical sectioning, with imaging results acquired from a wide range of live samples.