09:45 〜 10:15
▲ [15a-C31-3] [JSAP-OSA Joint Symposia 2016 Invited Talk] Hybrid light microscopy methods for multi-dimensional imaging of small animal models
キーワード：Light microscopy, Imaging speed, Optical sectioning
Light microscopy methods have been widely used in biomedical research. Different microscopy techniques have different advantages and disadvantages. For example, wide-field optical microscopy is known for its convenience and high imaging speed. However, it has no depth sectioning capability due to the missing axial high-frequency components in its 3D optical transfer function. It is therefore impossible to obtain 3D structures of specimen based on wide-field images stacks. Laser scanning microscopes (e.g., confocal microscopes), on the other hand, can provide the so-called optical sectioning capability and high axial resolution. While conventional confocal microscopy is a standard tool for 3D visualization of fixed samples, their slow scanning speed and excessive photobleaching make it less suitable for real-time in vivo imaging of live samples. We have developed a hybrid strategy to combine information information from both scanning microscopy and wide-field microscopy imaging modes. A prototype hybrid microscope has been designed and implemented. We have verified its imaging performances, such as reduced photobleaching, improved imaging speed, and improved optical sectioning, with imaging results acquired from a wide range of live samples.