The 63rd JSAP Spring Meeting, 2016

Presentation information

Print

Oral presentation

12 Organic Molecules and Bioelectronics » 12.7 Biomedical Engineering and Biochips

[19p-W331-1~16] 12.7 Biomedical Engineering and Biochips

Sat. Mar 19, 2016 1:45 PM - 6:00 PM W331 (W2・W3)

Koichiro Miyamoto(Tohoku Univ.), Takao Suda(Natl. Inst. of Tech.,Kagoshima Col.)

4:15 PM - 4:30 PM

[19p-W331-10] Polydopamine thin films as protein adhesion layer for surface plasmon enhanced fluorescence biosensors

〇mana toma1, keiko tawa1 (1.Kwansei Gakuin Univ.)

Keywords:Surface plasmon enhanced fluorescence,biosensor,polydopamine

In this contribution, a mussel inspired polydopamine (PDA) thin film is employed as a protein adhesive layer in surface plasmon enhanced fluorescence (SPF) biosensors, and detection of inflammatory maker Interleukin 6 (IL6) is performed. After a gold surface supporting surface plasmons is coated with PDA, adsorption kinetics of anti-IL6 antibody is taken by surface plasmon resonance spectroscopy. Sandwich immunoassay of IL6 using fluorescence labeled anti-IL6 detection antibody is carried out. The sensing performance of SPF biosensors using PDA coatings, including detection limit of IL6, will be presented.