[20p-P11-1~25] 12.6 Nanobiotechnology

▲ [20p-P11-1] Modification of Carbon-Based Materials with MPC Polymer Brush for Applications in Intracellular Nanosensors

〇(M1)Tinnlea Uen¹, Keiichiro Kushiro¹, Madoka Takai¹ (1.The Univ. of Tokyo)

Keywords:polymer brush,carbon electrode,Code-sharing session

Detection of local concentration of ions, metabolite and drugs in living cells is important for drug development in pharmaceutical companies. Recently, carbon-based electrochemical nanosensors have been developed, which enables such measurements. However, protein adsorption onto the carbon electrode surface results in lower sensitivity of the sensors. In this study, carbon-based materials were modified with poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) brushes, which have been shown to suppress protein adsorption, through surface-initiated atom transfer radical polymerization (SI-ATRP). MPC-modified cyclic olefin polymer (COP) and glassy carbon electrode surfaces were characterized by brush thickness, graft chain density, wettability and elemental composition. Overall, the results show successful modification of the carbon-based surfaces that may be applied to the nanosensors for the detection of the localized intracellular drug concentrations.