[20p-P11-1~25] 12.6 Nanobiotechnology

[20p-P11-15] Molecular recognition imaging using an amplitude-modulation atomic force microscope equipped with a photo-thermal excitation system

Takashi Nyu¹, Tetsu Ogawa¹, Rina Ohashi¹, 〇Tomohiro Hayashi^1,2 (1.Tokyo TECH, 2.RIKEN)

Keywords:Atomic force microscopy,molecular recognition,imaging

We developed a high speed imaging technique to map molecular recognition events by using an amplitude-modulation atomic force microscopy equipped with a photo-thermal excitation technique. In this work, we employed this method to image the molecular recognition events of streptavidin and biotin, which were immobilized on a substrate and probe, respectively. In this presentation, we discuss the principle of this technique, the optimization of the experimental conditions, and perspectives in future.