[20p-P12-1~31] 12.7 Biomedical Engineering and Biochips

△ [20p-P12-19] Optimization of a Sedimentation Hole for Separating Plasma from Whole Blood

〇(DC)CHIAKI KURODA¹, Ohki Yoshimichi^1,2, Ashiba Hiroki³, Fujimaki Makoto³, Awazu Koichi³, Tanaka Torahiko⁴, Makishima Makoto⁴ (1.ASE, Waseda Univ., 2.RIMST, Waseda Univ., 3.AIST, 4.Nihon Univ.)

Keywords:plasma separation,microfluidics,biosensor

In order to detect pathogens such as viruses or their antibodies in plasma efficiently, plasma has to be separated from whole blood by removing blood cells in advance. In this study, the shape of a hole to sediment blood cells was optimized by taking account of the Boycott effect, aiming at putting the hole in a microfluidic chip. As a result, it has become clear that the microfluidic chip with a truncated right conical sedimentation hole can separate about 5 μL of plasma with a purity higher than 99% when about 30 μL of human blood with hematocrit of 10% is retained in the hole only for 2 min.