[20p-P12-1~31] 12.7 Biomedical Engineering and Biochips

▲ [20p-P12-22] Study on peptide-functionalized single–walled carbon nanotube field effect transistor for aptasensor

〇Tung Thanh Nguyen¹, Trong Tue Phan¹, Thi Ngoc Lien Truong², Yasuhide Ohno³, Kenzo Maehashi⁴, Kazuhiko Matsumoto⁵, Manish Biyani¹, Yuzuru Takamura¹ (1.Japan Advanced Inst. of Sci. and Tech., 2.HUST, 3.Tokushima Univ., 4.Tokyo Univ. Agr. Tech., 5.Osaka Univ. ISIR)

Keywords:aptamer,SWCNT FET,peptide aptamer

Single-walled carbon nanotube field effect transistors (SWCNT FETs) have drawn much attention for biosensing application thanking to their quasi-one-dimensional SWCNT channel which is very small compared to the biomolecule dimension. However, short Debye-screening is a very severe drawback of SWCNT FET, which makes probe molecules that must be shorter than the Debye length. This issue could be resolved by using small receptor like DNA aptamers. In contrast with DNA aptamers that bind to complementary targets using entire sequence, peptide aptamers are smaller and only bind to their targets with single variable loop region. These distinct characteristics lead to reduction of the flexibility of peptide aptamers, and thus improving effectiveness. Accordingly, peptide aptamers appear as a strong bioreceptor candidate. In this work, we focused on studying peptide modification on SWCNT FET for biomolecule analysis.