[20p-P12-1~31] 12.7 医用工学・バイオチップ

▲ [20p-P12-22] Study on peptide-functionalized single–walled carbon nanotube field effect transistor for aptasensor

〇Nguyen Tung Thanh¹、Phan Trong Tue¹、Truong Thi Ngoc Lien²、Ohno Yasuhide³、Maehashi Kenzo⁴、Matsumoto Kazuhiko⁵、Biyani Manish¹、Takamura Yuzuru¹ (1.Japan Advanced Inst. of Sci. and Tech.、2.HUST、3.Tokushima Univ.、4.Tokyo Univ. Agr. Tech.、5.Osaka Univ. ISIR)

キーワード：aptamer,SWCNT FET,peptide aptamer

Single-walled carbon nanotube field effect transistors (SWCNT FETs) have drawn much attention for biosensing application thanking to their quasi-one-dimensional SWCNT channel which is very small compared to the biomolecule dimension. However, short Debye-screening is a very severe drawback of SWCNT FET, which makes probe molecules that must be shorter than the Debye length. This issue could be resolved by using small receptor like DNA aptamers. In contrast with DNA aptamers that bind to complementary targets using entire sequence, peptide aptamers are smaller and only bind to their targets with single variable loop region. These distinct characteristics lead to reduction of the flexibility of peptide aptamers, and thus improving effectiveness. Accordingly, peptide aptamers appear as a strong bioreceptor candidate. In this work, we focused on studying peptide modification on SWCNT FET for biomolecule analysis.