2016年第63回応用物理学会春季学術講演会

講演情報

一般セッション(ポスター講演)

12 有機分子・バイオエレクトロニクス » 12.7 医用工学・バイオチップ

[20p-P12-1~31] 12.7 医用工学・バイオチップ

2016年3月20日(日) 16:00 〜 18:00 P12 (屋内運動場)

16:00 〜 18:00

[20p-P12-7] Development of a pattering technique for enzyme-immobilized membranes using cross-linkable polymer

〇(DC)Youna Lee1、Horio Tomoko1、Okumura Koichi1,2、Iwata Tatsuya1,2、Takahashi Kazuhiro1,2、Ishida Makoto1,2、Sawada Kazuaki1,2 (1.Toyohashi Univ.、2.EIIRIS)

キーワード:biosensor,enzyme-immobilized membrane,cross-linkable polymer

In this research, we suggest a patterning technology of an enzyme-immobilized membrane consisted of cross-linkable polymer by using lithography method. The suggested technique will be applied to fabricate various membranes for the developing a multi-detection sensor which can simultaneously detect several kinds of neurotransmitters in brain. As a first step of the development, a layer of apyrase-containing cross-linkable polymer was fabricated on a 128×128 pixel ion-image sensor and experimentally demonstrated to monitor the distribution and dynamics of adenosine triphosphate (ATP) activity. Responsivity according to various concentrations of ATP solution were measured. And the output voltage distribution was determined with normal distribution curves for ATP solution. Based on these measured characteristics, we successfully achieved the sequential images according to ATP movement by the enzymatic reaction between apyrase in the patterned membrane and the dropped ATP.