12:00 PM - 12:15 PM
△ [21a-W621-12] Induction Effect on Plant Defence Gene Expression in Oryza sativa by Reactive Species Generated in Atmospheric Air Plasma
Keywords:Plasma agriculture,reactive oxygen species,induced resistance
Recently, plant activators, which induce systemic acquired resistance (SAR) in plants, are widely investigated in the agricultural field to replace synthetic organic pesticides. Biochemical effects of Atmospheric pressure plasma on living tissue, such as sterilization and growth promotion, are also widely investigated to develop application utilizing reactive oxygen species (ROS) and reactive nitrogen species (RNS). ROS and RNS analysis, such as ozone treatment or micro electrostatic atomized water particles, have been reported to relate SAR. Therefore, ROS and RNS generated by the atmospheric pressure plasma could also induce SAR.
In this work, we report induction effect on defense related genes by atmospheric pressure plasma. Two types of plasma jet using air and water has been developed. Rice seeds (Haenuki) are grown under dark at 28℃ for 2 days, and the wet germinated seeds are exposed to the plasma for 15 min. Plasma exposed seeds are then stored under dark at 28℃ for 2 days. Total RNA is isolated from leaves and roots. Gene expression was evaluated by microarray assay, which detect exhaustive gene expression over a few million kinds of genes. Highly induced genes of each samples were surveyed on Oryzabase, an integrated rice science database. The results shows that the defense related genes are expressed by the plasma exposure, which indicates composition of ROS and RNS induces the gene expression. Investigations on relation between chemical species and the induction effect will be discussed.
In this work, we report induction effect on defense related genes by atmospheric pressure plasma. Two types of plasma jet using air and water has been developed. Rice seeds (Haenuki) are grown under dark at 28℃ for 2 days, and the wet germinated seeds are exposed to the plasma for 15 min. Plasma exposed seeds are then stored under dark at 28℃ for 2 days. Total RNA is isolated from leaves and roots. Gene expression was evaluated by microarray assay, which detect exhaustive gene expression over a few million kinds of genes. Highly induced genes of each samples were surveyed on Oryzabase, an integrated rice science database. The results shows that the defense related genes are expressed by the plasma exposure, which indicates composition of ROS and RNS induces the gene expression. Investigations on relation between chemical species and the induction effect will be discussed.