[21p-W331-1~19] CS.2 3.7/12.6/12.7 Code-sharing session

[21p-W331-13] Measuremet of Reactive Species for the Development of Plasma-on-Chip

Junseok Oh^1,2, Shinya Kojima³, Akimitsu Hatta^1,2, Minoru Sasaki³, 〇Shinya Kumagai³ (1.Kochi Univ. Technol., 2.Center Nanotechnol., 3.Toyota Technol. Inst.)

Keywords:Plasma-on-Chip,UV absorption spectroscopy,Cell

Low-temperature atmospheric-pressure plasmas are getting importance in the biomedical field. To understand the interaction between the plasma and cells, we have suggested a brand new device “plasma-on-chip”. The plasma-on-chip device consists of microwells and microplasma sources which are fabricated together in a chip. Between the microwells and microplasma sources, there are micro through-holes. The plasma-on-chip device enables to culture cells in the microwells and to deliver plasma-generated reactive species to the cells through a gas-liquid interface formed at the micro through-hole(s). In the 76^th JSAP Autumn meeting, we reported the inactivation of Chlorella cells using the plasma-on-chip. It remained as an open question which plasma-generated reactive species were delivered into the microwells and inactivated the cells. In this study, we demonstrated the delivery of the reactive species by the analysis using in-situ UV absorption spectroscopy and analyzed the concentrations of reactive species.