4:00 PM - 4:15 PM
▼ [21p-W611-3] Biomolecules Patterning via Atmospheric Pressure Plasma Jet for the Development of Carbon Nanotube Microarrays
Keywords:atmospheric pressure plasma jet,carbon nanotube,biosensor
Microarray is a new technology which provides an efficient biomolecule identification technique over traditional technologies, such as ELISAs and Western blots, due to their ability of carrying out the multiplex detection of biomolecules on a single platform and minimal sample consumption. At present, carbon nanotubes (CNTs) are attracting materials for biosensor due to their outstanding chemical and physical properties. CNTs combine superior biomolecular recognition capabilities and unique physical properties of nanoscale that make them very promising for developing biosensor based on CNTs [1]. However, for biomolecules patterning three-dimesional (3D) surface of CNT microarrays, conventionally low-pressure plasmas driven by RF or microwaves require physical masks that make the process cumbersome, expensive and time consuming. Among the numerous plasma technologies, atmospheric pressure plasma jet (APPJ) are of intense interest for surface modification due to the advantages of no need vacuum conditions, technically simple and maskless functionalization [2].
In this work, we study the development of CNT microarrays via APPJ as shown in Fig.1. CNTs with a spacing of 50 µm and a dot size of 5 µm were synthesized by a combined thermal-plasma CVD devices, and constructed in dot array form for realizing the development of CNT microarrays. An ultrafine APPJ with a micro-capillary was utilized to functionalize amino groups on designated CNT spot without mask. Two-stage plasma treatments were conducted in order to functionalize CNT microarrays by ultrafine APPJ. The first plasma treatment stage was pretreatment. The CNT microarrays were pretreated 0.2 sec by a helium gas with a –500-V. The second plasma treatment stage was posttreatment. The CNT then posttreated 2 sec without bias by helium and ammonia gas mixture APPJ. After both pretreatment and posttreatment were performed to the designated spot of CNT microarrays, the samples were scanned automatically to the next designated spot by computer-controlled X–Y stage. Biotin-avidin system and antibody-antigen reaction of E. coli was used to assess the feasibility of biomolecule immobilization on CNT microarrays. The possibility of this technique for microarray applications was demonstrated by successfully functionalized CNT using ultrafine APPJ and biomolecules patterning on CNT microarrays.
In this work, we study the development of CNT microarrays via APPJ as shown in Fig.1. CNTs with a spacing of 50 µm and a dot size of 5 µm were synthesized by a combined thermal-plasma CVD devices, and constructed in dot array form for realizing the development of CNT microarrays. An ultrafine APPJ with a micro-capillary was utilized to functionalize amino groups on designated CNT spot without mask. Two-stage plasma treatments were conducted in order to functionalize CNT microarrays by ultrafine APPJ. The first plasma treatment stage was pretreatment. The CNT microarrays were pretreated 0.2 sec by a helium gas with a –500-V. The second plasma treatment stage was posttreatment. The CNT then posttreated 2 sec without bias by helium and ammonia gas mixture APPJ. After both pretreatment and posttreatment were performed to the designated spot of CNT microarrays, the samples were scanned automatically to the next designated spot by computer-controlled X–Y stage. Biotin-avidin system and antibody-antigen reaction of E. coli was used to assess the feasibility of biomolecule immobilization on CNT microarrays. The possibility of this technique for microarray applications was demonstrated by successfully functionalized CNT using ultrafine APPJ and biomolecules patterning on CNT microarrays.