[5a-S22-1~8] 8.6 Plasma life sciences

[5a-S22-2] Selective antitumor effect of plasma-activated medium produced by microwave excited plasma treatment

〇Yohei Takahashi^1,3, Yusuke Taki¹, Keigo Takeda², Hiroshi Hashizume³, Hiromasa Tanaka³, Masaru Hori³ (1.Nikon Corporation, 2.Meijo Univ., 3.Nagoya Univ.)

Keywords:atmospheric pressure plasma, Plasma medicine

Recently, atmospheric pressure plasma has been widely developed for the applications on various fields, such as synthesis approaches, surface modification, sterilization, etc. Especially, cancer therapy using atmospheric pressure plasma is one of the most attractive applications. The culture medium irradiated with the atmospheric pressure plasma was called Plasma-Activated Medium (PAM), which exhibited the selective apoptotic cell death of cancer cells. In this study, we have demonstrated the antitumor effect of medium induced by irradiation of atmospheric pressure plasma with high plasma density and compared the cell survival between cancer and normal cells, which showed that the selective apoptotic cell death was achieved. Additionally, the basic diagnostics of the plasma and the analysis of the PAM were performed and the relation with the antitumor effects was discussed. This transition is the intense systems emitted by low temperature plasmas containing even a small amount of H₂O. The selective apoptotic cell death effect by treatment with PAM produced by atmospheric pressure plasma irradiation was confirmed. The survival of cancer cell after incubation in PAM was greatly lower than that of normal cell was. The productions of H₂O₂ and NO₂^- by irradiation of high density plasma were detected by the colorimetric assay. The synergistic effect of H₂O₂ and NO₂^- in PAM is considered to affect the proliferation of cancer cells.