[6p-PB5-1~12] 12.6 Nanobiotechnology

[6p-PB5-6] Unsaturated lipid concentration dependence for phase separation at a lipid bilayer suspended over microwells

Koki Harada¹, Yuto Nakatani¹, Sho Tamura¹, Azusa Oshima², Hiroshi Nakashima², 〇Koji Sumitomo¹ (1.University of Hyogo, 2.NTT BRLs.)

Keywords:Lipid bilayer, Phase separation, Raft-like structure

The localization of liquid-ordered (L_o) and liquid-crystalline (L_a) phase domains is investigated on a silicon substrate with a microwell array. It was known that unsaturated lipid is condensed at the suspended membrane, and saturated lipid and cholesterol are excluded. It was difficult to remain the L_o phase domain at the suspended membrane. In this study, we have tried to control the phase separation at the suspended membrane by changing the ratio of unsaturated lipid.