[6p-PB6-1~18] 12.7 Biomedical Engineering and Biochips

[6p-PB6-18] Design Optimization of Weir Structure-Based Filters for White Blood Cell Sorting

〇(M1)Tsuyoshi Ono¹ (1.Osaka Univ.)

Keywords:micro fluidic device, cell sorting

This report presents the cell sorting capability of the weir structured-based filters in the designed microfluidic chip. Sorting and separation of cells from a heterogeneous mixture is a vital process in any cell-related research. The biophysical characteristics of the cell such as morphology, size, density, and dielectric susceptibility are commonly utilized in a label-free sorting method but often lacks specificity. The use of labels or biological markers is common alternative but succeeding processes can have an influence on the cellular phenotype. In this study, the cell sorting capability is associated to the deterministic cell rolling of related to the rapid formation and rapid dissociation of adhesive bond on a contact surface. The weir structure is subjected to different cells available to test its compatibility and with the ultimate goal of sorting the type of cells in a blood, specifically, white blood cells. The design has been further modified based on the results.
With this result, an alternative sorting method can be realized that can be utilized for low concentration cell volume; a common downside of commercialized machines and protocols. To enhance the sorting capability, optimized coating will be needed which can interact with the different integrin present on the cell. In the future, the optimized design shall be integrated in a centrifugal platform for high throughput processing.